Figure 5.

AdMYH6 effects on rabbit cardiac myocyte maximal calcium-activated (pCa 4.5) isometric contractile function. A) Maximal isometric tension generation of skinned (1% Triton X-100) cardiac myocytes was not affected by AdMYH6 gene transfer. B) Myofilament calcium sensitivity did not differ between control (•) and AdMYH6-treated (○) myocytes. P, tension development; P₀, maximal tension development; P/P₀, relative tension. C, D) Maximal rate of isometric cross-bridge cycling was faster in AdMYH6-treated cardiac myocytes vs. control myocytes (1.40±0.07 s⁻¹, n=16 vs. 1.16±0.07 s⁻¹, n=16). All values are means ± se. *P < 0.05 vs. control; two-tailed t test.