Figure 7.

AdMYH6 gene transfer augments failing human cardiac myocyte contraction. A) Fluorescent image of a single human cardiac myocyte demonstrates successful adenovirus-mediated gene transfer of GFP. Image was taken 48 h after gene transfer. B) Representative sarcomere length shortening measurements in human failing ventricular cardiac myocytes. α-MyHC gene transfer enhanced the contractile amplitude of failing human cardiac myocytes paced at 1 Hz. C) Summary of data. HF amplitude = 23.9 ± 7.8 nm, n = 10; HF+AdGFP amplitude = 21.8 ± 5.2nm, n = 5; HF + AdMYH6 amplitude = 78.4 ± 16.5 nm, n = 6. All values are means ± se. *P < 0.05 vs. control, two-tailed t test. Actual P values are shown in figure. Human myocyte data were analyzed by 1-way ANOVA as there were 3 groups to compare: HF, HF + AdGFP, and HF + AdMYH6.