Figure 3.

Inhibition of SOD and catalase increases ROS generation and reduces cell growth of the transformed cells. A and B, the transformed cells were treated with the SOD inhibitor 2-ME, the catalase inhibitor 3-AT, or both for 48 h. The levels of O₂^•− and H₂O₂ were determined then in these cells by DHE and CM-H₂DCFDA staining, respectively, followed by flow cytometry. C, increasing ROS generation by SOD or catalase inhibition inhibits cell growth of the transformed cells. D, increasing ROS generation by SOD or catalase inhibition inhibits anchorage-independent growth of the transformed cells.