Skip to main content
. Author manuscript; available in PMC: 2014 Jun 9.
Published in final edited form as: Cancer Res. 2010 Jun 1;70(12):5127–5135. doi: 10.1158/0008-5472.CAN-10-0007

Figure 5.

Figure 5

Altered expression of the NF-κB family proteins in the transformed cells. A, the protein levels of IKK subunits IκBα, pIκBα, p65, and RelB were determined by Western blotting. Actin was used as a loading control. B, expression and proteolytic processing of p105 and p100 were determined by Western blotting. C, top, NF-κB DNA binding activity was determined by EMSA; bottom, expression and nuclear translocation of NF-κB p50 protein in the BEAS-2B (left) and transformed (right) cells as determined by immunofluorescence staining using p50 antibody. D, increasing ROS generation of the transformed cells by SOD or catalase inhibition reduces NF-κB DNA binding activity. The transformed cells were treated with both SOD and catalase inhibitors (lane 3), control miRNA (lane 4), or both SOD and catalase miRNAs for 48 h. Nuclear proteins were prepared and incubated with the labeled DNA probe containing NF-κB binding site.