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. Author manuscript; available in PMC: 2015 Mar 1.
Published in final edited form as: Stem Cell Res. 2013 Dec 16;12(2):441–451. doi: 10.1016/j.scr.2013.12.003

Figure 2.

Figure 2

Wnt signaling is inhibitory to the generation of CD235+CD41+ early hematopoietic progenitors from human ESCs. (A) Flow cytometric analyses of CD235 vs. CD41 on day 9 non-adherent cells treated with DKK1 (Dkk) or Wnt3a starting on day 4 of differentiation. (B) Cell yields from the entire culture (adherent and non-adherent cells) or non-adherent cells treated as described in A. (C) QRT-PCR gene expression analyses of lineage specific genes in control and DKK1 treated cells. (D) Day 9 non-adherent cells treated as described in A assayed for progenitor potential in methylcellulose and Mega-cult colony assays.