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. Author manuscript; available in PMC: 2015 Mar 1.
Published in final edited form as: Stem Cell Res. 2013 Dec 16;12(2):441–451. doi: 10.1016/j.scr.2013.12.003

Figure 3.

Figure 3

Canonical Wnt signaling inhibits hematopoiesis. (A) Flow cytometric analyses of CD235 vs. CD41 on day 9 non-adherent cells treated on day 4 of differentiation with sFRP1, Wnt5a, GSK3β inhibitor (GSK), and GSK3β inhibitor + Wnt5a starting. (B) Cell yields for the entire culture (adherent and non-adherent) and non-adherent cells treated as described in A. (C) QRT-PCR expression analyses of lineage specific genes in non-adherent cells treated as described in A.