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. Author manuscript; available in PMC: 2015 Mar 1.
Published in final edited form as: Stem Cell Res. 2013 Dec 16;12(2):441–451. doi: 10.1016/j.scr.2013.12.003

Figure 4.

Figure 4

Wnt signaling inhibits the expansion or survival of CD235+CD41+ CMPs. (A) The left flow cytometric profile shows sorted day 9 CD235+CD41+ H9-derived CMPs. The right profiles show these cells cultured for 7 days in hematopoietic cytokines ± DKK1 (Dkk) or Wnt3a and analyzed for markers of megakaryocytes (CD41 vs. CD42), erythrocytes (CD41 vs. CD235), and myeloid cells (CD45 vs. CD18). (B) Cell counts of the cells treated as described in A at days 9, 13 and 16 compared to the untreated (Control) cells. (C) QRT-PCR expression analyses of lineage specific genes in day 4 mesoderm, day 9 sorted CD235+CD41+ cells, and cells treated with DKK1/Wnt3a as described in A. (D) Sorted day 9 CD235+CD41+ cells as described in A plated in methylcellulose and Mega-cult based colony assays with the addition of DKK1 (Dkk) or Wnt3a.