FIG. 4.

Isolation of spheres from the spiral ganglion of early postnatal mice. Quantification of floating cell colonies with a maximum diameter ≥40 μm that form from dissociated spiral ganglion cells in the presence of growth factors and without growth factors (vehicle) after 7 days in a nonadherent petri dish. Data are the mean±SD from three independent experiments; **p<0.01 compared with vehicle (bovine serum albumin [BSA]). bFGF, basic fibroblast growth factor; EGF, epidermal growth factor; IGF-1, insulin-like growth factor-1; HS, heparan sulfate.