FIG. 7.

Spiral ganglion spheres conserve the expression of the neural stem/progenitor cell marker nestin after propagation. (A, B) Nestin expression is abundantly expressed in the cytoplasm of primary sphere cells. Nestin expression can also be detected in secondary (C) and tertiary spheres (D). (E) Quantification of nestin expression in primary spiral ganglion-derived spheres and subsequent sphere generations. Data are the mean±SD from three independent experiments. Nuclei in B–D are visualized with DAPI (blue). Scale bar=60 μm in A, 100 μm in B–D.