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. 2014 Jun 1;3(3):110–116. doi: 10.1089/biores.2014.0008

FIG. 1.

FIG. 1.

Engraftment capacity of hematopoietic stem and progenitor cells (HSPCs) is not affected by a short in vitro culture. (A) Phenotype of transplanted cells. Plots are gated on the population indicated on top. (B) Engraftment as measured by percentage of human CD45 expressing cells in peripheral blood of humanized NSG mice transplanted with different cell doses of either fresh or cultured HSPCs isolated from umbilical cord blood (UCB). (C) Different cell lineages in peripheral blood of mice transplanted with 1.5×105 noncultured HSPCs isolated from UCB (top) or 1.5×105 cultured HSPCs (bottom). Myeloid cells (left), B cells (middle), and T cells (right) were gated within human CD45+ cells. (D) Contribution of different cell lineages in peripheral blood at 18 weeks after transplantation. Percentages are gated within human CD45+ cells. (E) Numbers of long-term-HSC (hCD45+CD34+CD38−CD90+CD45RA−CD49f+) present in bone marrow (BM) of NSG recipients transplanted with different cell doses of either fresh or cultured HSPCs isolated from UCB. Data are represented as mean±standard deviation (SD) in (B, D, E). 50k, 0.5×105 cells; 150k, 1.5×105cells. HSPCs were isolated from a pool of seven UCB donors, three mice per group.