FIG. 1.
Differentiation potential of sorted Sox1-green fluorescent protein (Sox1-GFP) mouse embryonic stem cells (mESCs). (A) Time course analysis showing the percentage of Sox1-GFP positive cells during neural differentiation from day 0 (d0) to day 7 (d7). Error bars represent±SEM with n=3 independent experiments. (B) Brightfield pictures of sorted Sox1-GFP+ (left panels), Sox1-GFP− cells (middle panels) and unsorted cells (right panels) at day 7 (top) and at day 13 (bottom). Scale bars, 100 μm. (C) Quantitative reverse-transcription polymerase chain reaction (RT-qPCR) showing the expression of the pluripotency marker Oct3/4, the epithelial marker Keratin (K)18 and the mesodermal marker Brachyury, expressed as ΔΔCt values, in GFP+ (black lines) and GFP− (dark gray lines) from day 0 (d0) to day 13 (d13), and in unsorted cells (light gray indicator) at day 13. Error bars represent±SEM with n=3 independent experiments. d5p, day 5 pre-sorting; ns, not significant. *p<0.05, ***p<0.001. Ct, cycle threshold.