FIG. 2.
Neural induction of sorted Sox1-GFP cells. (A) RT-qPCR analyses showing the expression of Sox1, Nestin, βIII Tubulin, NCAM, O4, and GFAP markers during differentiation, expressed as ΔΔCt values, in GFP+ (black lines) and GFP− (dark gray lines) from day 0 (d0) to day 13 (d13) and in unsorted cells (light gray indicator) at day 13. Error bars represent±SEM with n=3 independent experiments. d5p, day 5 pre-sorting; ns, not significant. *p<0.05. (B) Western blot analysis showing the expression of Nestin and βIII-Tubulin during differentiation. GAPDH was used for normalization. (C) Immunocytochemistry showing expression of Nestin, βIII Tubulin, MAP2, and GFAP in Sox1-GFP+ (left), Sox1-GFP− (middle), and unsorted (right) cells at day 13 of differentiation. Hoechst 33342 (Life Technologies) was used to counterstain nuclei. Scale bars, 100 μm.