Skip to main content
. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: Traffic. 2014 Mar 7;15(5):516–530. doi: 10.1111/tra.12159

Figure 5. Binding of IKKβ to SNAP23 is reduced following E. coli exposure.

Figure 5

(A) RBLs were incubated with or without (control) E. coli at an MOI of 10,000 for 2h. The cells were harvested and stimulated for 30min with 1μM PMA/1μM Ionomycin at a concentration of 2×106 cells/ml, lysed and SNAP23 was immunoprecipitated from 1mg of total protein. Immunoprecipitated samples and 40μg of whole cell lysates were analyzed by Western blot with anti-SNAP23 and -IKKβ antibodies. (B) Graph represents the mean band intensity ratio of IKKβ to total SNAP23 ± the standard deviation of 3 independent experiments. The IKKβ/SNAP23 ratio of the control population was considered 100%. Asterisks denote statistically significant p values, where ** ≤ 0.01.