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. Author manuscript; available in PMC: 2014 Jun 9.
Published in final edited form as: Cell Rep. 2014 Apr 24;7(3):762–773. doi: 10.1016/j.celrep.2014.03.056

Figure 4. Derepression of repetitive elements in prg-1 mutants is cured by daf-2.

Figure 4

(A) Late-generation prg-1 mutants show a mean increase in transposon expression, compared to the expression of transposons in prg-1; daf-2 double mutants. (B) Density plot of the transposon expression changes shown in Figure 4A reveal increased expression of a subset of transposon sequences in late-generation prg-1 animals that is not seen in prg-1; daf-2 double mutants. (C) Simple repeats are upregulated in late-generation prg-1 mutants. (D) Simple repeats upregulated in prg-1 mutants are repressed in late generation prg-1; daf-2 mutants. (E–H) cDNA prepared from RNA was hybridized to microarrays revealed upregulation of tandem repeat tracts expression in late-generation prg-1 mutants but not in late generation prg-1; daf-2 mutants (E and G), as confirmed by RT-PCR analysis for late-generation wild-type as well as early-(E), and late-(L) generation mutant strains (F and H). F and H show expression of tandem repeats corresponding to E and G, respectively. (I) Genome-wide plots of 101 longest tandem repeat tracts defined by visually scanning the C. elegans genome in 70 kb sliding windows. Typically, tandem repeats display increased expression in late-generation prg-1(tm872) and prg-1(n4357) single mutants but silencing in prg-1 double mutants with daf-2 alleles e1368, e1370 or m41.