Skip to main content
. Author manuscript; available in PMC: 2014 Jun 9.
Published in final edited form as: Nat Genet. 2013 Feb 3;45(3):330–333. doi: 10.1038/ng.2525

Figure 2.

Figure 2

Effects of PSR1 and PSR2 on small RNA biogenesis in Arabidopsis. (a) RNA blotting showing lower levels of two representative miRNAs, one ta-siRNA (ASR5D8) and one hc-siRNA (SiR1003) in wild-type (Col-0) and three independent PSR1-expressing Arabidopsis lines. (b) Transcript abundance of pri-miRNAs from various miRNA genes in wild-type and PSR1- or PSR2-expressing lines as determined by RT-PCR. Error bars, s.e.m. from two experimental replicates. (c) RNA blotting showing lower levels of pre-miR164b and pre-miR166a in two PSR1-expressing lines. The two bands probably both represent pre-miRNA species. Relative expression levels were analyzed using the prominent upper bands. (d) Accumulation of three representative ta-siRNAs (ASRP255, ASRP1151 and ASR5D8 from TAS1, TAS2 and TAS3 loci, respectively), two miRNAs and one hc-siRNA (SiR1003) in wild-type and three independent PSR2-expressing transgenic lines. Changes in siRNA abundance were not evident in the transgenic line PSR2-18 because of the low expression level of PSR2. U6 served as a loading control. Numbers below the blots represent the relative abundance of the small RNAs with the levels in wild-type plants set to 1.