FIG 4 .

Early protection induced by primary BCG ΔureC::hly Δpdx1 vaccination depends on vitamin B₆ supplementation of mice. (A) Schematic design of protection studies. (B) Impact of vitamin B₆ on bacterial burdens in lungs and spleen after M. tuberculosis aerosol infection of mice that received primary vaccination only. Groups of mice received vitamin B₆ (VB6)-enriched diet as indicated starting 2 weeks prior to vaccination until M. tuberculosis challenge. Shown are means ± SEM (n = 5 or 6) analyzed using one-way ANOVA and Tukey’s posttest. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. Data from one experiment representative of two independent biological replicates are shown.