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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: Nat Commun. 2014;5:3071. doi: 10.1038/ncomms4071

Figure 2. Staged differentiation of hiPSCs into human EpSCs.

Figure 2

a. An outline of the protocol used to differentiate hiPSCs to EpSCs and then mature keratinocytes. b. Morphologies of hiPSCs, hiPSC-derived EpSCs (hiPSC-EpSCs, obtained at day 18 after differentiation) and hiPSC-derived mature keratinocytes (hiPSC-keratinocytes, obtained at day 45 after differentiation). Scale bar, 100 µm. c-e. Flow cytometric analysis of CD200+/ITGA6+, KRT15+ and KRT14+ cells at day 0 and 18 during the differentiation. f-h. Quantitation of CD200+/ITGA6+, KRT15+ and KRT14+ cells by flow cytometric analysis. Data shown are mean ± SD of cell percentage from three independent experiments. i. qPCR analysis of OCT3/4, NANOG, KRT5, KRT8, KRT14, KRT15, LamB3, involucrin and filaggrin expression in hiPSC-derived cells at different stages of differentiation. Samples collected at day 0, day 11, day 18, day 25 and day 30 after differentiation were used for qRT-PCR analysis. Data shown are mean ± SD of the expression from three independent experiments.