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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: Nat Commun. 2014;5:3071. doi: 10.1038/ncomms4071

Figure 3. Colony formation capacity of hiPSC-derived EpSCs.

Figure 3

a. Colony formation assays. Representative dishes of hiPSC-derived mature keratinocytes (hiPSC-keratinocytes), hiPSC-derived CD200+/ITGA6+ cells (hiPSC-EpSCs), passaged normal keratinocytes from adult skin (passage 3), and hiPSC-derived CD200−/ITGA6+ cells (hiPSC-CD200−/ITGA6+), cultured for 3 weeks on 3T3 fibroblast feeder cells. The dishes were stained with H&E. Representative images from three independent experiments. b. qPCR analysis of pluripotent stem cell markers in the hiPSC-derived CD200+/ITGA6+, CD200+/ITGA6− and CD200−/ITGA6+ cells. The pluripotent stem cell markers used are OCT3/4, NANOG and REX1. Data shown are mean ± SD of the expression from three independent experiments. c. qPCR analysis of the keratinocyte specific genes in hiPSC-derived CD200+/ITGA6+ and CD200+/ITGA6− cells. Data shown are mean ± SD of the expression from three independent experiments. d. Morphology of colonies formed by CD200+/ITGA6+ and CD200+/ITGA6− cells. Scale bar in upper panel, 100um; scale bar in lower panel, 20 um.