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. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: Alcohol Clin Exp Res. 2014 May 30;38(6):1550–1558. doi: 10.1111/acer.12439

Fig. 1. Effect of ethanol exposure on single strand DNA damage measured by comet assay in HBECs.

Fig. 1

Monolayers of human bronchial epithelial cells (HBECs) were pretreated for 1 hr with diallyl disulfide (DADS; 10 μM) and further treated for 3 hr with ethanol (EtOH; 80 mM), cigarette smoke extract (CSE; 5%) and co-exposure condition. Hydrogen peroxide (H202; 0.1 mM) was used as positive control. Exposure to ethanol caused significant DNA damage, which was reduced significantly by DADS exposure. Values represent mean ± S.E.M. of (100–150) cells scored. Experiment was performed 3 independent times.