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. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: Alcohol Clin Exp Res. 2014 May 30;38(6):1550–1558. doi: 10.1111/acer.12439

Fig. 4. Effect of ethanol and cigarette smoke on M1dG levels in HBECs and C57BL/6 mice whole lung tissue homogenate.

Fig. 4

Lysate of human bronchial epithelial cells (HBECs) were pretreated for 1 hr with diallyl disulfide (DADS; 10 μM) and further exposed to ethanol (EtOH; 80 mM), cigarette smoke extract (CSE; 5%) and co-exposure condition for 6h and crude homogenates of mouse lung tissue were assayed using indirect ELISA for M1dG levels. Ethanol alone and in combination with cigarette smoke significantly increased M1dG levels. Ethanol exposure significantly increased M1dG levels, which were reduced significantly by DADS supplement. Values represent mean ± S.E.M. of n=4 mice and of n=3 independent experiments for HBECs.