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. Author manuscript; available in PMC: 2014 Aug 1.
Published in final edited form as: Cell Signal. 2014 Apr 18;26(8):1657–1667. doi: 10.1016/j.cellsig.2014.04.011

Figure 4. Increased kinase activities of NDR1 variants do not correlate with changes in hMOB1 and hMOB2 binding.

Figure 4

(A-C) Lysates of Cos-7 cells expressing the indicated combinations of HA-tagged NDR1 forms and wild-type myc-tagged hMOB variants were analysed by immunoprecipitation (IP) using anti-HA 12CA5 antibody. Immunocomplexes were analysed by Western blotting using anti-myc 71D10 (top panel) and anti-HA 42F13 antibody (top middle panel). Input lysates were analysed with anti-myc 9E10 (bottom middle panel) and anti-α-tubulin YL1/2 antibody (bottom panel). Molecular weights are shown. Noteworthy, hyperactive NDR1-PIF displays decreased binding to hMOB1A/B, while complex formation with hMOB2 is not impaired.