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. Author manuscript; available in PMC: 2014 Aug 1.
Published in final edited form as: Cell Signal. 2014 Apr 18;26(8):1657–1667. doi: 10.1016/j.cellsig.2014.04.011

Figure 5. In contrast to NDR1-PIF, wild-type NDR1 requires endogenous hMOB1A/B for auto- and trans-phosphorylation activities.

Figure 5

(A) U2-OS cells stably expressing tetracycline-regulated shRNA directed against hMOB1A/B were incubated for 72 hours without (-) or with (+) tetracycline before being transfected with indicated HA-NDR1 forms overnight. Subsequently, cells were subjected to immunoprecipitation (IP) using anti-HA 12CA5 antibody. Complexes and input lysates were analysed by Western blotting using indicated antibodies. Molecular weights are shown. An unspecific signal is indicated by an asterisk. (B) In parallel, immunocomplexes were subjected to kinase assays. Cumulative data from three independent experiments with two replicates per experiment are shown. Error bars indicate standard deviations.