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. Author manuscript; available in PMC: 2014 Jun 9.
Published in final edited form as: Nat Methods. 2010 May 30;7(7):519–520. doi: 10.1038/nmeth.1465

Figure 1.

Figure 1

Relationship between PrPSc concentration and PMCA rounds required for detection. (a) Aliquots with the indicated amounts of PrPSc were subjected to serial rounds of PMCA (144 cycles each) using standard conditions, and PrPSc was detected by western blotting using the 3F4 antibody. All samples, except the normal brain homogenate (NBH) used as a migration control were digested with proteinase K (PK). (b) Western blots from five independent experiments (as the example shown in a) were analyzed by densitometry, and the last detectable signal after each PMCA round was plotted, yielding a standard calibration curve to estimate PrPSc amounts in hamster samples. Error bars, s.e.m. (n = 5).