Figure 1. ATXR5 and ATXR6 selectively methylate the histone H3 variant H3.1.
A. Alignment of the canonical histone H3 variants H3.1 and H3.3 from A. thaliana (At) and human (Hs). Identities are dark-shaded. B. In vitro HKM assay using recombinant chromatin containing plant histone H3.1 or plant histone H3.3 as substrates and various histone methyltransferases from A. thaliana. C. Michaelis–Menten plot of initial velocity vs peptide substrate concentration. The kcat and Km values for the H3.1 and H3.3 peptides are shown as inlet. Errors bars represent the standard deviations of three independent experiments each performed in triplicates with three different batches of RcATXR5. D. In vitro HKM assay using recombinant chromatin containing plant histone H3.1, plant histone H3.3 or plant histone H3.3 T31A.