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Relative level of BAFF and APRIL mRNA determined by real-time PCR. cDNA was constructed using RNA isolated from Peyer’s Patches (PP), mesenteric lymph node (MLN), and spleen of wild-type (WT) and MyD88−/− mice at 0, 1, and 2 days after oral RASV administration. Expression level of BAFF and APRIL mRNA was normalized by GAPDH. Data are representative of 3 independent experiments.
