Figure 2.

3′-Exonuclease (SVPDE) degradation of singly (left, 3′-CAC BAT ACG) and doubly modified (right, 3′-CAC BAB ACG) C5-functionalized LNA and reference strands. Nuclease degradation studies were performed in magnesium buffer (50 mM Tris-HCl, 10 mM Mg²⁺, pH 9.0) by using 3.3 μM ONs and 0.03 U of SVPDE. Data depicted in the left panel have been previously reported in ref (22).