Table 2. Thermodynamic Parameters for Formation of Duplexes Modified with C5-Functionalized LNA Monomersa.
| +complementary
DNA |
+complementary
RNA |
||||||
|---|---|---|---|---|---|---|---|
| ON | sequence | ΔG298 [ΔΔG298] (kJ/mol) | ΔH [ΔΔH] (kJ/mol) | –T298ΔS [Δ(T298ΔS)] (kJ/mol) | ΔG298 [ΔΔG298] (kJ/mol) | ΔH [ΔΔH] (kJ/mol) | –T298ΔS [Δ(T298ΔS)] (kJ/mol) |
| D1 | 5′-GTG ATA TGC | –42 | –314 | 271 | –36 | –278 | 241 |
| D2 | 3′-CAC TAT ACG | –42 | –314 | 271 | –39 | –293 | 254 |
| L1 | 5′-GTG ALA TGC | –47 [−5] | –297 [+17] | 250 [−21] | –49 [−13] | –309 [−31] | 260 [+19] |
| L2 | 3′-CAC LAT ACG | –46 [−4] | –332 [−18] | 286 [+15] | –47 [−8] | –331 [−38] | 283 [+29] |
| L3 | 3′-CAC TAL ACG | –49 [−7] | –332 [−18] | 283 [+12] | –50 [−11] | –340 [−47] | 290 [+36] |
| K1 | 5′-GTG AKA TGC | –49 [−7] | –350 [−36] | 301 [+30] | –53 [−17] | –424 [−146] | 371 [+130] |
| K2 | 3′-CAC KAT ACG | –49 [−7] | –349 [−35] | 300 [+29] | –49 [−10] | –367 [−74] | 317 [+63] |
| K3 | 3′-CAC TAK ACG | –52 [−10] | –372 [−58] | 319 [+48] | –57 [−18] | –414 [−121] | 357 [+103] |
| M1 | 5′-GTG AMA TGC | –51 [−9] | –390 [−76] | 339 [+68] | –52 [−16] | –386 [−108] | 334 [+93] |
| M2 | 3′-CAC MAT ACG | –50 [−8] | –394 [−80] | 344 [+73] | –51 [−12] | –398 [−105] | 347 [+93] |
| M3 | 3′-CAC TAM ACG | –51 [−9] | –360 [−46] | 309 [+38] | –51 [−12] | –367 [−74] | 316 [+62] |
| N1 | 5′-GTG ANA TGC | –51 [−9] | –353 [−39] | 302 [+31] | –51 [−15] | –324 [−46] | 272 [+31] |
| N2 | 3′-CAC NAT ACG | –49 [−7] | –362 [−48] | 313 [+42] | –52 [−13] | –364 [−71] | 312 [+58] |
| N3 | 3′-CAC TAN ACG | –52 [−10] | –361 [−47] | 309 [+38] | –52 [−13] | –325 [−32] | 272 [+18] |
| O1 | 5′-GTG AOA TGC | –47 [−5] | –337 [−23] | 290 [+19] | –46 [−10] | –337 [−59] | 291 [+50] |
| O2 | 3′-CAC OAT ACG | –44 [−2] | –322 [−8] | 278 [+7] | –43 [−4] | –366 [−73] | 322 [+68] |
| O3 | 3′-CAC TAO ACG | –46 [−4] | –324 [−10] | 278 [+7] | –44 [−5] | –340 [−47] | 296 [+42] |
| P1 | 5′-GTG APA TGC | –45 [−3] | –334 [−20] | 289 [+18] | –45 [−9] | –327 [−49] | 282 [+41] |
| P2 | 3′-CAC PAT ACG | –43 [−1] | –324 [−10] | 281 [+10] | –43 [−4] | –351 [−58] | 308 [+54] |
| P3 | 3′-CAC TAP ACG | –44 [−2] | –339 [−25] | 294 [+23] | –43 [−4] | –365 [−72] | 321 [+67] |
| Q1 | 5′-GTG AQA TGC | –45 [−3] | –346 [−32] | 301 [+30] | –45 [−9] | –347 [−69] | 302 [+61] |
| Q2 | 3′-CAC QAT ACG | –45 [−3] | –411 [−97] | 371 [+100] | –46 [−7] | –377 [−84] | 331 [+77] |
| Q3 | 3′-CAC TAQ ACG | –43 [−1] | –287 [+27] | 243 [−28] | –43 [−4] | –360 [−67] | 317 [+63] |
| S1 | 5′-GTG ASA TGC | –37 [+5] | –317 [−3] | 280 [+9] | –39 [−3] | –333 [−55] | 294 [+53] |
| S2 | 3′-CAC SAT ACG | –39 [+3] | –380 [−66] | 342 [+71] | –42 [−3] | –359 [−66] | 316 [+62] |
| S3 | 3′-CAC TAS ACG | –40 [+2] | –380 [−66] | 339 [+68] | –40 [−1] | –355 [−62] | 315 [+61] |
a
Parameters were determined from thermal denaturation curves, which were recorded as described in Table 1. ΔΔG298, ΔΔH, and Δ(T298ΔS) are calculated relative to reference duplexes D1:D2, D1:R2, and D2:R1.