Table 3. Discrimination of Mismatched DNA/RNA Targets by Singly Modified LNAs and Reference ONs^a.

		DNA: 3′-CAC TBT ACG				RNA: 3′-CAC UBU ACG
		Tm	ΔTm			Tm	ΔTm
ON	sequence	A	C	G	T	A	C	G	U
D1	5′-GTG ATA TGC	29.5	–16.5	–8.0	–15.5	27.0	<−17.0	–4.5	<−17.0
L1	5′-GTG ALA TGC	34.5	–18.0	–11.0	–16.0	36.5	–19.0	–8.0	–18.5
K1	5′-GTG AKA TGC	36.5	–20.0	–15.5	–18.5	38.0	–20.5	–13.5	–22.0
M1	5′-GTG AMA TGC	36.5	–20.0	–11.5	–18.5	36.5	–18.5	–9.5	–20.0
N1	5′-GTG ANA TGC	37.5	–19.0	–12.0	–17.5	40.0	–18.5	–11.5	–22.5
O1	5′-GTG AOA TGC	34.0	–20.5	–16.5	–18.0	33.0	–20.0	–9.5	–20.0
P1	5′-GTG APA TGC	33.5	–21.5	–17.0	–20.5	32.5	–20.5	–11.5	–19.5
Q1	5′-GTG AQA TGC	30.5	–18.0	–13.0	–16.5	31.0	–19.5	–10.0	–20.0
S1	5′-GTG ASA TGC	24.0	–11.5	–10.0	–11.0	25.0	–15.0	–9.0	<−15.0
V1	5′-GTG AVA TGC	23.0	–7.5	–10.0	–7.5	23.0	<−13.0	–10.5	<−13.0
W1	5′-GTG AWA TGC	21.0	+6.0	–7.0	+3.0	25.0	<−15.0	<−15.0	<−15.0
X1	5′-GTG AXA TGC	17.0	+4.5	±0.0	+3.5	15.0	<−5.0	<−5.0	<−5.0
Y1	5′-GTG AYA TGC	19.0	–1.0	–4.0	–2.5	25.0	<−15.0	–8.0	<−15.0
Z1	5′-GTG AZA TGC	24.0	–10.0	<−14.0	–9.5	25.5	–1.5	<−15.5	<−15.5

^aFor experimental conditions and sequences, see Table 1. ΔT_m = change in T_m value relative to fully matched ON:DNA or ON:RNA duplex (B = A). Data for L1/K1/N1/Q1/S1 against mismatched RNA have been previously published in ref (22).