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. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: Alcohol Clin Exp Res. 2014 May 29;38(6):1646–1653. doi: 10.1111/acer.12417

Figure 4. Requirements of Ca2+ signaling for ethanol-induced apoptosis.

Figure 4

HTR-8/SVneo (A-B) or SW.71 (C-D) cytotrophoblast cells were preincubated with vehicle or the indicated Ca2+ signaling inhibitors, as in Fig. 3, and then treated with vehicle or 50 mM ethanol. After 60 min, cells were assessed for apoptosis by the TUNEL (A, C) or Annexin V-binding (B, D). Experiments were repeated three times and the averages with error bars indicating the SEM are shown. *, p < 0.05 compared to vehicle treatment.