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. Author manuscript; available in PMC: 2015 Mar 14.
Published in final edited form as: Circ Res. 2014 Jan 29;114(6):957–965. doi: 10.1161/CIRCRESAHA.114.303411

Figure 5. Deletion of YAP induces a subset of cell cycle arrest gene expression without affecting expression of SM-specific genes.

Figure 5

A. Total RNA was harvested from E15.5 control (open bars) YAP KO (hatched bars) dorsal aorta and qRT-PCR was performed to assess gene expression as indicated. The level of gene expression in control vessels was set to 1 (red line). N=5–7 embryos per genotype. *p<0.05. B. A representative western blot using protein lysates from E15.5 control or YAP KO dorsal aorta tissue is shown. C. Immunoblot signals were normalized to α-tubulin loading control then expressed relative to signals from control aortae (set to 1, red line). N=4 embryos per genotype. *p<0.05. D. Empty retrovirus or retrovirus encoding YAP was transduced into rat primary aortic SMCs and then Gpr132 expression was analyzed by western blotting. An arrow points to the endogenous YAP band.