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. Author manuscript; available in PMC: 2014 Jun 9.
Published in final edited form as: Circ Res. 2013 Aug 20;113(8):986–996. doi: 10.1161/CIRCRESAHA.113.301296

Figure 3. Depletion of Brg1 or Brm attenuates transcriptional activation of adhesion molecules by pro-inflammatory stimuli.

Figure 3

(A) Promoter luciferase fusion constructs for ICAM-1, VCAM-1 and E-selectin genes were transfected into HUVECs with either siRNA targeting Brg1 or Brm or scrambled siRNA (SCR) as indicated followed by treatment with TNF-α. Luciferase activities were expressed as NRLU. *, p<.05 (B–E) HUVECs were transfected with siBrg1, siBrm, or SCR followed by treatment with TNF-α. mRNA (B) and protein (C) levels of CAMs were assessed by qPCR and Western. Similar experiments were performed in HAECs (D, E). *, p<.05 (F) HUVECs were transfected with siBrg1, siBrm, or SCR followed by treatment with TNF-α. Leukocyte adhesion assay was performed as described under Methods. Data are shown as mean +SD of triplicates and are representative of one experiment out of three performed.