Skip to main content
. 2014 Feb 18;23(13):3579–3595. doi: 10.1093/hmg/ddu068

Figure 2.

Figure 2.

C9ORF72 colocalizes with endosomal Rabs; Rab1, Rab5, Rab7 and Rab11 in neuronal cell lines. (A) Neuro2a cells were fixed and immunostained with anti-C9ORF72 antibodies (red) and either anti-Rab7 or anti-Rab1 antibodies (green), or transfected with constructs encoding either GFP-Rab5 or GFP-Rab11 for 48 h, followed by DAPI staining (blue). The white arrows indicate regions of colocalization between C9ORF72 and the respective Rabs. Scale bar: 10 μm, applied to all fields. Insets demonstrate higher magnification (×100) of the areas highlighted to illustrate colocalization with vesicular structures. (B) Co-immunoprecipitation followed by western blotting revealed that Rab7 and Rab11 are pulled down using anti-C9ORF72 antibodies, and that C9ORF72 is pulled down using anti-Rab1 antibodies. Control immunoprecipitations using an isotype-matched, irrelevant IgG antibody were negative, indicating no cross-reactivity with the antibodies used. 1% input also shown.