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. 2014 Apr 1;2(3):229–239. doi: 10.1002/mgg3.58

Figure 5.

Figure 5

Cell proliferation and viability of NOTCH3. (A) Stable cells were incubated with (closed argyles) or without (open squares) tetracycline and harvested on days 0, 1, 3, and 5 for each of the nine stable cell lines (WT-17, WT-21, WT-38, G840E-5, G840E-21, G840E-36, T900P-14, T900P-29, and T900P-33). Each data point represents the mean number of cells with the standard deviation from four independent experiments. (B) Stable cells were incubated with or without tetracycline for 3 days. Cell viability was determined using the WST-1 reagent. The control indicates T-REx 293 cells that were not transfected. Values represent the means with the standard deviation from three independent experiments and are shown as the percentage of the total number of cells without tetracycline treatment. (C) The cell viability demonstrated in (B) was converted to a percentage of the mean value relative to that of stable cells expressing wild-type NOTCH3. Each data point represents the mean and standard deviation from nine cell lines. ***P < 0.001.