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. 2014 Feb 11;2(3):261–272. doi: 10.1002/mgg3.67

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© 2014 The Authors. Molecular Genetics & Genomic Medicine published by Wiley Periodicals, Inc.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Minigene assay of THAP1 splicing. (A) Segments of THAP1 were cloned into the multiple cloning site (MCS) of the pET01 exon trap vector, which contains a short stretch of a eukaryotic phosphatase gene (E) 3′ to the eukaryotic strong long terminal repeater promoter of the Rous Sarcoma Virus (RSV). A second segment of E is followed by a polyadenylation site (pA). (B) The results of three wild-type and three c.71+9C>A transfections showed that the C>A mutation exerted significant effects on splicing patterns in HEK293 cells (*P < 0.05, for all).