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. 2014 May 28;11:100. doi: 10.1186/1743-422X-11-100

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Copyright © 2014 Haugh et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Daxx is induced by type I IFNs. (A) Immunoblot analysis of Daxx protein levels (antibody from Sigma) after human (h) IFN-α (1000 U/ml) treatment of HeLa cells for the indicated time points. (B) Real-time PCR showing Daxx mRNA levels in DF-1 cells following treatment with chicken (ch) IFN-α for the indicated time points. Values were normalized to GAPDH. (C) Immunoblot analysis of Daxx protein levels following chicken (ch) IFN-α treatment of DF-1 cells for the indicated times. β-actin (antibody from Sigma) is shown as a loading control. Molecular-weight markers, in kilodaltons, are shown to the left of each blot.