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. 2014 May 16;14:132. doi: 10.1186/1471-2229-14-132

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Copyright © 2014 Lancilli et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Estimation of the growth constant (k_G) dependent on sulfate of the yeasts expressing the Brassica juncea sulfate transporters. The duplication times (dt) of the complemented yeast cells were calculated by fitting the equation A₆₀₀(t) = A₆₀₀(t₀) e^kt to the experimental data reported in Additional file 5. k_G was estimated by expressing the growth rates (dt^-1) of complemented yeasts as a function of sulfate concentrations in the media, and by fitting the Michaelis-Menten equation to the data. Data points and error bars are means and SE of two experiments run in triplicate (n = 6).