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. 2014 May 29;14:379. doi: 10.1186/1471-2407-14-379

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Copyright © 2014 Wang et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Protein expressions in PND50 rats. Protein expressions of ER-α, ER-β, SRC-1, SRC-2 and SRC-3 from mammary gland extracts of 50 day old rats exposed prepubertally via lactating dams treated with BPA or/and genistein, and SO. (A) Top panel depicts the Western blots of mammary gland proteins from SO (lanes 1, 5, 9, 13, 17 and 21), BPA (lanes 2, 6, 10, 14, 18 and 22), genistein (lanes 3, 7, 11, 15, 19 and 23) and BPA plus genistein (lanes 4, 8, 12, 16, 20 and 24) -treated groups with the appropriate antibodies shown on the left. For each treatment group, n = 6. (B) Densitometric values of Western blots from PND50 rats were reported as a percentage of the controls ± SEM: *p ≤ 0.05 compared with controls; **p ≤ 0.05 compared with genistein; ***p ≤ 0.0001 compared with BPA.