Table 2.
Primary and secondary antibodies for immunoblot analysis
|
Primary Antibodies | |||||
|---|---|---|---|---|---|
| Antibody name | Supplier | Product number | Source | Dilution | Molecular weights |
| Akt-1 |
Cell Signaling |
2938 |
Rabbit |
1:1000 |
60 kDa |
| Akt-2 |
Cell Signaling |
5239 |
Mouse |
1:1000 |
60 kDa |
| Akt-3 |
Cell Signaling |
3788 |
Rabbit |
1:1000 |
60 kDa |
| p-Akt |
Cell Signaling |
4060 |
Rabbit |
1:2000 |
60 kDa |
| Cleaved Caspase-3 |
Cell Signaling |
9661 |
Rabbit |
1:1000 |
17/19 kDa |
| Cleaved Caspase-9 |
Cell Signaling |
9507 |
Rabbit |
1:1000 |
17 kDa |
| PARP |
Cell Signaling |
9542 |
Rabbit |
1:1000 |
89/116 kDa |
| p-Bad |
Cell Signaling |
9296 |
Mouse |
1:1000 |
23 kDa |
| p21 |
Cell Signaling |
2946 |
Mouse |
1:1000 |
21 kDa |
| PTEN |
Cell Signaling |
9552 |
Rabbit |
1:1000 |
54 kDa |
| ER-α |
R&D System |
MAB57151 |
Mouse |
1:2000 |
65 kDa |
| ER-β |
Santa Cruz |
sc-8974 |
Rabbit |
1:200 |
56 kDa |
| SRC-1 |
BD Biosciences |
612378 |
Mouse |
1:250 |
165 kDa |
| SRC-2/TIF2 |
BD Biosciences |
610984 |
Mouse |
1:250 |
160 kDa |
| SRC-3/AIB-1 |
BD Biosciences |
611105 |
Mouse |
1:250 |
160 kDa |
| GAPDH |
Cell Signaling |
2118 |
Rabbit |
1:1000 |
38 kDa |
| Secondary Antibodies | |||||
| Antibody Name |
Supplier |
Product Number |
Source |
Dilution |
|
| Anti-Rabbit IgG HRP |
R&D System |
HAF008 |
Goat |
1:2000 |
|
| Anti-Mouse IgG HRP | R&D System | HAF007 | Goat | 1:2000 | |