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. 2014 May 20;8:33. doi: 10.1186/1752-153X-8-33

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Copyright © 2014 Gaber et al.; licensee Chemistry Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Comparison of HPLC analyses of the reaction components in a crude reaction mixture from the synthesis of N-lauroyl-N-methyl-glucamide by isocratic method using UV (A) and RI (B) detectors, and the developed gradient method using Evaporative Light Scattering Detector ELSD (C). Solutes: MEG: 1; amide: 2; lauric acid: 3; amide-ester: 4. Isocratic method: mobile phase: methanol: water: TFA 75:25:0.3 v/v ; flow rate 1 ml min⁻¹ and UV wavelength set at 210 nm.