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ELSD optimisation for HPLC analysis of solutes. Methyl laureate was used as analyte for the optimization experiment. The Signal over Noise ratio (S/N) was monitored regarding the following factors: the detector temperature (A), the gas flow rate in the detector (B), and the flow rate of the mobile phase (C). Standard conditions are mobile phase flow rate of 1 ml/min, 1.3 L/min gas flow to the detector, detector temperature is 38°C, and gain of the detector is set to 1.
