Table 1.
Chromatographic conditions adopted for gradient HPLC separation of reaction components in the enzymatic synthesis of N-lauroyl N-methyl glucamide
|
Mobile phase |
Solvent A = |
Water: trifluoroacetic acid (0.05% w/w) |
|
| |
Solvent B = |
Methanol |
|
|
Gradient |
Time (min) |
% Solvent A |
% Solvent B |
| |
0 |
25 |
75 |
| |
5 |
25 |
75 |
| |
10 |
5 |
95 |
| |
10 |
5 |
95 |
| |
5 |
25 |
75 |
| |
10 |
25 |
75 |
|
Flow rate |
1 ml/min |
|
|
|
Column |
LiChrospher® 100 RP-18 (5 μm) (LiChroCART® 125-4 HPLC cartridge) Merck, Darmstadt, Germany |
||
|
Column temperature |
40°C |
|
|
|
Detection |
ELSD (Alltech 3300, Alltech Associates, USA) |
||
|
Injection volume |
5 ul |
|
|
|
ELSD settings |
|
|
|
|
Temperature |
38°C |
|
|
|
Gas flow (air) |
1.3 L/min |
|
|
|
Gain |
1 |
|
|
| Run time | 40 min | ||