Table 3. RT-PCR confirmation of 15 genes used in CD8+ Tcell activation prediction models.
| Symbol | UniGene | TaqMan assay | Day | Model | Pearson r | P-value |
|---|---|---|---|---|---|---|
| RGS1 | Hs.75256 | Hs0017526_m1 | 3 | ClaNC | 0.8924 | 0.0005 |
| CD69 | Hs.208854 | Hs0015399_m1 | 3 | ClaNC | 0.8837 | 0.0007 |
| ALDH3B1 | Hs.523841 | Hs00997594_m1 | 3 | ClaNC | 0.8117 | 0.0002 |
| CXCR7 | Hs.471751 | Hs00664172_s1 | 3 | ClaNC | 0.788 | 0.0068 |
| C1QB | Hs.8986 | Hs00608019_m1 | 3 | ClaNC | 0.7803 | 0.0077 |
| ASGR2 | Hs.16247 | Hs00154160_m1 | 7 | ClaNC | 0.7202 | 0.0025 |
| JUN | Hs.525704 | Hs99999141_s1 | 3 | ClaNC | 0.7184 | 0.0193 |
| CXCR7 | Hs.471751 | Hs00664172_s1 | 7 | ClaNC | 0.7078 | 0.0032 |
| ATP6V1E1 | Hs.517338 | Hs00762211 S1 | 3 | ClaNC | 0.6841 | 0.0049 |
| ASGR2 | Hs.16247 | Hs00154160 m1 | 3 | ClaNC | 0.6056 | 0.0167 |
| SLC2A6 | Hs.244378 | Hs00214042_m1 | 7 | ClaNC, DAMIP | 0.5494 | 0.0339 |
| MEF2A | Hs.268675 | Hs00271535_m1 | 7 | ClaNC | 0.5423 | 0.0368 |
| CTBP1 | Hs.208597 | Hs00179922_m1 | 7 | DAMIP | 0.4634 | 0.0819 |
| ITGAL | Hs.174103 | Hs00158238_m1 | 7 | DAMIP | 0.4517 | 0.091 |
| EIF2AK4 | Hs.412102 | Hs00383836_m1 | 7 | ClaNC, DAMIP | 0.4124 | 0.1266 |
The Pearson r is calculated for the log2-fold change microarray data versus the relative RT-PCR measurements on either Day 3/Day 0 or Day 7/Day 0 with data points from each of the subjects samples assayed.