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. 2014 Jun 3;7:259. doi: 10.1186/1756-3305-7-259

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Copyright © 2014 Liu et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Dot-blot hybridization, A and B was used to test the cDNA library of NT strain, C and D was used to test the cDNA library of SH strain. A: The probes that came from subtractive PCR products of the NT strain were used to test the cDNA library of NT strain; B: The probes that came from unsubtractive PCR products of the SH strain were used to test the cDNA library of NT strain; C: The probes that came from subtractive PCR products of the SH strain were used to test the cDNA library of SH strain; D: The probes that came from unsubtractive PCR products of the NT strain were used to test the cDNA library of SH strain. Each clone was blotted in the same place in two identical membranes. a were negative control; b were positive control.