Figure 4.

Biological regulated by KLF11 and its chromatin binding partners. Genes significantly regulated by wild type KLF11 and its mutants compared to empty vector were analyzed for enrichment of biological processes by an ontological approach. A threshold of 3 genes and a p-value of less than 0.05 by Fisher’s Exact Test were required to be considered significantly regulated by the transcription factor and its mutants. (A) Venn diagram of biological processes demonstrates that the A347S mutant causes the largest number of genome-wide effects with significant alterations in 150 processes, 93 of which are unique to the mutant. Only one biological process, lipid cellular metabolism, is dependent on the combinatorial effects of all three chromatin coupling systems. The top biological processes were computed for each of the proteins compared to empty vector. The pie charts for (B) Wild type KLF11, (C) A347S mutant, (D) Δ486 mutant, and (E) EAPP mutant display the top ten scoring biological processes for each with enrichment percentage displayed. These results indicate that the effects of KLF11 coupling to chromatin co-factors impact on a large number of biological functions.