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. 2014 May 7;21(1):39. doi: 10.1186/1423-0127-21-39

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Copyright © 2014 Tripathi et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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UV-B irradiation induces photodegradation and attenuation of the neuroprotective effects of RF in human neuroblostoma SH-SY5Y cell lines. (A) LC-MS/MS spectrum of parent RF (376 Da) and (B) two major photoproducts P1 (360.4 Da) and P2 (343.2 Da) after UV-B (0.6 mW/cm 2 for 1 h) irradiation. (C) RF treatment significantly inhibits OGD induced LDH release compared to that of UV-B-RF treatment (D) RF treatment significantly rescues cell viability compared to that of UV-B-RF treatment in Human neuroblostoma SHSY5Y cell line. The average of three independent experiments performed in triplicate is shown. Statistical significance was analyzed by ANOVA. Values are the mean ± SEM *p < 0.05 and **p < 0.01 vs. RF treated and control group.