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. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: Nat Genet. 2014 Apr 28;46(6):635–639. doi: 10.1038/ng.2963

Figure 2. DCM-associated RAF1 mutants activate AKT.

Figure 2

Representative immunoblot with total lysates from HEK293 cells expressing wild-type (WT), HCM-associated RAF1 mutant proteins (p.Ser257Leu, p.Val263Ala and p.Leu613Val) and DCM-associated RAF1 mutant proteins (p.Thr310Ala and p.Ala237Thr) that were stimulated with EGF for 15 min and were probed with anti-phospho-ERK1/2, anti-ERK1/2, anti-phospho-AKT and anti-AKT antibodies. Expression levels were normalized to respective total proteins and expressed as Relative Expression compared to level in the WT cells. GAPDH levels were used as loading control. Data are mean values ± SD of four independent experiments assayed in duplicates. *p <0.05, **p <0.01 or ***p <0.001 vs WT.