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. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: Nat Genet. 2014 Apr 28;46(6):635–639. doi: 10.1038/ng.2963

Figure 3. DCM-associated RAF1 mutants require Braf for Akt activation.

Figure 3

Representative immunoblots with total lysates from mouse embryonic fibroblasts (MEFs) from Raf1 and Braf knockout mice (Raf1−/− and Braf−/−, respectively) expressing wild-type (WT) and two representative DCM mutants (p.Pro332Ala and p.Leu603Pro) that were probed with anti-phospho-Akt and anti-Akt antibodies. Expression levels were normalized to respective total proteins and expressed as Relative Expression compared to level in the WT cells. Gapdh levels were used as loading control. Data are mean values ± SD of four independent experiments assayed in duplicates. *p <0.05 vs WT.