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. Author manuscript; available in PMC: 2014 Jun 9.
Published in final edited form as: Biochem J. 2013 Jan 1;449(1):143–150. doi: 10.1042/BJ20120869

Figure 2. Concentration dependence of deoxyHb blockade of ankyrin binding to KI-IOVs.

Figure 2

KI-IOVs (30 μg/ml) were incubated in an air-tight 2-ml plastic tube with increasing concentrations of haemoglobin at room temperature. The system was then either left untreated (oxygenated) or mildly flushed with humidified argon for 1 h. Ankyrin (final concentration of 6 μg/ml) was added through the rubber stopper and its binding to KI-IOVs was measured as described in Figure 1. Results are means ± S.D., n = 3.