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. Author manuscript; available in PMC: 2014 Jun 9.
Published in final edited form as: Mol Microbiol. 2011 Jul 12;81(4):1078–1091. doi: 10.1111/j.1365-2958.2011.07754.x

Figure 3. RTT109 is required for nicotinamide induced switching.

Figure 3

(A) Western blot of wild-type white cells (HLY3879) untreated, nicotinic acid treated (25mM), and nicotinamide (25mM) treated. H3K56Ac and H3 protein levels were probed after approximately six hours of growth at 30°C in YEPD media. (B) FACS plots of wild-type white cells grown in liquid SCD culture in the presence or absence of 25mM nicotinamide. (C) Quantitative switching assay of wild type (HLY3555) and rtt109 (HLY3998) in the presence of 25mM nicotinamide. Adjoining FACS plots are of representative nicotinamide treated colonies of either wild type (HLY3555) or rtt109 (HLY3998). For figure (C), the SCD without NAM switching frequency data are from Figure 1B with wild type n=1801 and rtt109 n=1351. Switching data are from at least two independent tests. n are the total number of colonies counted; error bars are differences in phenotypic scores between tests. Dashed lines are added for clarity.