Table 2. Pre-steady-state kinetic parameters for interaction of hOGG1 with oxoG-substratea.
Rate constants | Temperature | ||||
10°C | 15°C | 20°C | 25°C | 30°C | |
k 1, M−1s−1 | (1.3±0.7)×107 | (2.2±1.0)×107 | (1.7±0.8)×107 | (1.9±1.0)×107 | (2.0±1.0)×107 |
k -1, s−1 | 230±80 | 390±50 | 360±20 | 410±70 | 520±120 |
k 2, s−1 | 8.3±3.8 | 15.6±4.2 | 26.6±6.0 | 53.5±20.3 | 72.4±31.4 |
k -2, s−1 | 6.1±0.2 | 11.8±0.2 | 19.6±1.7 | 37.9±11.1 | 40.5±12.5 |
k 3, s−1 | 0.087±0.017 | 0.12±0.03 | 0.19±0.07 | 0.4±0.1 | 0.3±0.1 |
k -3, s−1 | 0.011±0.001 | 0.018±0.001 | 0.007±0.002 | 0.02±0.003 | 0.002±0.001 |
k 4, s−1 | 0.005±0.001 | 0.007±0.001 | 0.014±0.002 | 0.026±0.003 | 0.041±0.007 |
k 5, s−1 | 0.0006±0.0001 | 0.0012±0.0001 | 0.001±0.0001 | 0.001±0.001 | 0.0037±0.007 |
The errors indicated are ±1 St. Dev.